Ano Oni Kyoushi ga Boku no Ane ni Naru n desu ka? centers on a boy who is initially overjoyed at the news of him having an older sister due to his father remarrying to a woman who has a daughter, until he finds out that his new sister is actually his school's strict PE teacher, Shina. With his father going off to live with his new wife, the boy will live with alone with his new sister. In their new life together, the boy finds out that Shina at work is completely different from Shina at home: clumsy, scared of insects, and kind.